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Updated Practical 3 Processing 16S rRNA amplicon data (markdown)
authored
Jan 13, 2020
by
Ben Francis
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Practical-3-Processing-16S-rRNA-amplicon-data.md
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@@ -34,6 +34,7 @@ Now start up R, and we'll install the new packages:
> install.packages("devtools")
> library("devtools")
> devtools::install_github("benjjneb/dada2", ref="v1.14")
# this step will take a while
> install.packages("dada2",
repos = NULL,
type = "source",
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@@ -42,5 +43,12 @@ Now start up R, and we'll install the new packages:
### 1.2 Processing our data with DADA2
First let's go back to our data directory
> setwd("/bioinf/home/your_username/day_1/16S_amplicon/demultiplexed")
> setwd("/bioinf/home/your_username/day_1/16S_amplicons/demultiplexed")
Then start dada2:
> library(dada2)
The first parts of the process are now just preparing the files and file names.