Practical-3-Processing-16S-rRNA-amplicon-data.md

@@ -181,7 +181,7 @@ Make some basic diversity plots and rarefaction curves
 
 Now let's visualise the composition of our sample communities using basic barplots. For this, we first need to calculate relative abundance
 
-    physeq_herschel_rel <- transform_sample_counts(physeq_herschel, function(otu) otu/sum(out))
+    physeq_herschel_rel <- transform_sample_counts(physeq_herschel, function(otu) otu/sum(otu))
     physeq_herschel_rel
 
 First let's plot phylum-level composition