Updated Practical 3 Processing 16S rRNA amplicon data (markdown) authored by Ben Francis's avatar Ben Francis
......@@ -136,8 +136,7 @@ library(RColorBrewer)
plotTaxon <- function(seqtab, taxtable, taxon, min_abund, taxonomicLevel) {
seqtab <- data.frame(seqtab)
colnames(seqtab) <- paste(taxtable[, 1], taxtable[, 2], taxtable[, 3], taxtable[, 4],
taxtable[, 5], taxtable[, 6], as.character(1:length(taxtable[, 1])), sep="-")
colnames(seqtab) <- paste(taxtable[, 1], taxtable[, 2], taxtable[, 3], taxtable[, 4], taxtable[, 5], taxtable[, 6], as.character(1:length(taxtable[, 1])), sep="-")
seqtab <- seqtab[, grep("Chloroplast|Mitochondria", colnames(seqtab), invert=TRUE)]
seqtab <- seqtab/rowSums(seqtab)
seqtab1 <- seqtab[, sapply(seqtab, function(x) max(x)) >= min_abund]
......@@ -158,7 +157,8 @@ seqtab.m$Class <- sapply(strsplit(seqtab.m$variable, "-"), "[[", 3)
seqtab.m$Order <- sapply(strsplit(seqtab.m$variable, "-"), "[[", 4)
seqtab.m$Family <- sapply(strsplit(seqtab.m$variable, "-"), "[[", 5)
seqtab.m$Genus <- sapply(strsplit(seqtab.m$variable, "-"), "[[", 6)
seqtab.m <- cbind(seqtab.m, replicate(1,seqtab.m[colnames(seqtab.m) == taxonomicLevel]))
seqtab.m <- cbind(seqtab.m, replicate(1,seqtab.m[colnames(seqtab.m) ==
taxonomicLevel]))
colnames(seqtab.m) <- c(colnames(seqtab.m)[1:length(colnames(seqtab.m)) -1], "displayLevel")
nb.cols <- length(seqtab) - 1
mycolours <- rep(brewer.pal(12, "Paired"), ceiling(nb.cols/12))
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