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Update Practical 3b Processing 16S rRNA reads extracted from PacBio long reads authored by Taylor Priest's avatar Taylor Priest
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Practical-3b-Processing-16S-rRNA-reads-extracted-from-PacBio-long-reads.md
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...@@ -17,6 +17,7 @@ Great, once you have your 16S rRNA gene sequences, we can then analyse them usin ...@@ -17,6 +17,7 @@ Great, once you have your 16S rRNA gene sequences, we can then analyse them usin
Before we begin using DADA2, we need to tell our bash profile which version of R we want to use. Thankfully, the IT department previously set up a specific version of R that already has DADA2 and most of the necessary dependencies already installed, which saves us a lot of time. Run the following lines of code to direct your profile to the correct version of R and then start Rstudio: Before we begin using DADA2, we need to tell our bash profile which version of R we want to use. Thankfully, the IT department previously set up a specific version of R that already has DADA2 and most of the necessary dependencies already installed, which saves us a lot of time. Run the following lines of code to direct your profile to the correct version of R and then start Rstudio:
$ export RSTUDIO_WHICH_R=/opt/software/R/R-4.0.3/bin/R $ export RSTUDIO_WHICH_R=/opt/software/R/R-4.0.3/bin/R
$ source ~/.bashrc
$ rstudio $ rstudio
### 3.1 Processing our data with DADA2 ### 3.1 Processing our data with DADA2
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