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Updated Practical 3 Processing 16S rRNA amplicon data (markdown)
authored
Jan 13, 2020
by
Ben Francis
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Practical-3-Processing-16S-rRNA-amplicon-data.md
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@@ -24,7 +24,6 @@ Now, just to check which R we'll be using, run:
$ which R
You should see that the path is to the new R in your miniconda directory.
Now it's time to download DADA2. This we'll do by downloading it from github:
$ git clone https://github.com/benjjneb/dada2.git
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@@ -32,9 +31,16 @@ Now it's time to download DADA2. This we'll do by downloading it from github:
Now start up R, and we'll install the new packages:
$ R
> install.packages("devtools")
> library("devtools")
> devtools::install_github("benjjneb/dada2", ref="v1.14")
> install.packages("dada2",
repos = NULL,
type = "source",
dependencies = c("Depends", "Suggests","Imports"))
### 1.2
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### 1.2 Processing our data with DADA2
First let's go back to our data directory
> setwd("/bioinf/home/your_username/day_1/16S_amplicon/demultiplexed")