... | ... | @@ -104,7 +104,7 @@ Now we need to submit the script to Slurm with: |
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$ sbatch slurm-submit.sh
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Now you can run `squeue` and see if your job is running! Also you'll see a new file has been created for the output that would otherwise print to screen. Check this file to make sure you don't have any errors. Run time for GTDB-tk, especially the `de_novo_wf`, is pretty long (after all it has to compare your MAGs to a database of over 20,000 other genomes - check out the run-time we've reserved in the slurm script!), so carry on with the stuff below and you can take a look at the GTDB-tk outputs later today (`classify_wf` should finish today), or tomorrow.
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Now you can run `squeue` and see if your job is running! Also you'll see a new file has been created for the output that would otherwise print to screen. Check this file to make sure you don't have any errors. Run time for GTDB-tk, especially the `de_novo_wf`, is pretty long (after all it has to compare your MAGs to a database of over 30,000 other genomes - check out the run-time we've reserved in the slurm script!), so carry on with the stuff below and you can take a look at the GTDB-tk outputs later today (`classify_wf` should finish today), or tomorrow.
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It's also possible to load the phylogenetic tree output from GTDB-tk into arb, but it's a bit of a faff. We prepared an example of what it looks like for you to explore; it's in the `marmic_NGS2021/results/day_4/gtdbtk` directory. There's an arb database in there you can view (you first have to go to an arb server `$ ssh arb-X` (put in an number from 1 to 3 in place of the X), then run `arb` from the command line) and open the `MarMic-gtdbtk-example.arb` database.
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